Journal Article

Increase in Ascorbate Content of Transgenic Tobacco Plants Overexpressing the Acerola (<i>Malpighia glabra</i>) Phosphomannomutase Gene

Adebanjo A. Badejo, Hani A. Eltelib, Kazunari Fukunaga, Yukichi Fujikawa and Muneharu Esaka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 2, pages 423-428
Published in print February 2009 | ISSN: 0032-0781
Published online January 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn206
Increase in Ascorbate Content of Transgenic Tobacco Plants Overexpressing the Acerola (Malpighia glabra) Phosphomannomutase Gene

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  • Biochemistry
  • Molecular and Cell Biology
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Phosphomannomutase (PMM; EC 5.4.2.8) catalyzes the interconversion of mannose-6-phosphate to mannose-1-phosphate in the Smirnoff–Wheeler pathway for the biosynthesis of l-ascorbic acid (AsA). We have cloned the PMM cDNA from acerola (Malpighia glabra), a plant containing an enormous amount of AsA. The AsA contents correlate with the PMM gene expression of the ripening fruits and leaves. The PMM activities in the leaves of acerola, tomato and Arabidopsis correlate with their respective AsA contents. Transgenic tobacco plants overexpressing the acerola PMM gene showed about a 2-fold increase in AsA contents compared with the wild type, with a corresponding correlation with the PMM transcript levels and activities.

Keywords: Acerola (Malpighia glabra); Ascorbic acid; Enzyme activity; Phosphomannomutase; Phosphomannose isomerase; Transgenic tobacco

Journal Article.  3071 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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