Journal Article

Mitogen-Activated Protein Kinase Cascade in the Signaling for Polyamine Biosynthesis in Tobacco

Eun-Kyoung Jang, Kwang-Hyun Min, Su-Hyun Kim, Seung-Hee Nam, Shuqun Zhang, Young Cheol Kim, Baik Ho Cho and Kwang-Yeol Yang

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 3, pages 658-664
Published in print March 2009 | ISSN: 0032-0781
Published online January 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp009
Mitogen-Activated Protein Kinase Cascade in the Signaling for Polyamine Biosynthesis in Tobacco

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Expression of NtNEK2DD, a constitutively active mutant of NtMEK2, activates endogenous salicylic acid-induced protein kinase (SIPK) and wounding-induced protein kinase (WIPK), and leads to several stress/defense responses in tobacco. In this study, we used ACP (annealing control primer)-based differential display reverse transcription–PCR to isolate the downstream effectors mediated by the NtMEK2–SIPK/WIPK cascade. The arginine decarboxylase gene (ADC), which is involved in plant putrescine biosynthesis, was one of nine differentially expressed genes. When compared with NtMEK2KR plants, NtMEK2DD transgenic plants exhibited a significant increase in ADC and ODC (ornithine decarboxylase) transcript levels, as well as in putrescine and its catabolite, γ-aminobutyric acid, following SIPK/WIPK activation. Taken together, these results suggest that the NtMEK2–SIPK/WIPK cascade is involved in regulating polyamine synthesis, especially putrescine synthesis, through transcriptional regulation of the biosynthetic genes in tobacco.

Keywords: Arginine decarboxylase; ACP-based DDRT-PCR; NtMEK2–SIPK/WIPK cascade; Polyamine biosynthesis.

Journal Article.  2625 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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