Journal Article

The <i>Chlamydomonas</i> Hatching Enzyme, Sporangin, is Expressed in Specific Phases of the Cell Cycle and is Localized to the Flagella of Daughter Cells Within the Sporangial Cell Wall

Takeaki Kubo, Shinsuke Kaida, Jun Abe, Tatsuaki Saito, Hideya Fukuzawa and Yoshihiro Matsuda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 3, pages 572-583
Published in print March 2009 | ISSN: 0032-0781
Published online January 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp016
The Chlamydomonas Hatching Enzyme, Sporangin, is Expressed in Specific Phases of the Cell Cycle and is Localized to the Flagella of Daughter Cells Within the Sporangial Cell Wall

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The timely breakdown of the extracellular matrix by proteolytic enzymes is essential for development, morphogenesis and cell proliferation in plant and animal cells. Sporangin of the unicellular green alga Chlamydomonas reinhardtii that mediates breakdown of the sporangial cell wall to liberate the daughter cells after cell division is characterized as a subtilase-like serine protease. The sporangin gene is specifically transcribed during S/M phase in a synchronized vegetative cell cycle. In immunoblot analyses using a polyclonal antibody raised against the sporangin polypeptide, the enzyme is synthesized after mitotic cell division and accumulated in the daughter cells before hatching. Immunofluorescence analyses showed that sporangin is localized to the flagella of the daughter cells within the sporangial cell wall, and released into the culture medium. The data suggest that sporangin is released from flagella concurrently with the digestion of sporangial cell wall, and then the daughter cells are hatched from the sporangia in the Chlamydomonas vegetative cell cycle.

Keywords: Cell cycle; Chlamydomonas reinhardtii; Flagella; Hatching enzyme; Subtilase-like serine protease.

Journal Article.  7307 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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