Journal Article

A Cellulose Synthase-Containing Compartment Moves Rapidly Beneath Sites of Secondary Wall Synthesis

Raymond Wightman, Robin Marshall and Simon R. Turner

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 3, pages 584-594
Published in print March 2009 | ISSN: 0032-0781
Published online February 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp017
A Cellulose Synthase-Containing Compartment Moves Rapidly Beneath Sites of Secondary Wall Synthesis

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  • Biochemistry
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The woody secondary walls of plants represent the major sites of cellulose deposition. The polymerization of cellulose occurs at the plasma membrane by the secondary wall cellulose synthase complex (CSC). In the long, cylindrical cells that make up the xylem, secondary wall deposition is confined to discrete regions of the cell, and yellow fluorescent protein (YFP)-labeled CSCs are also localized to these regions. Using fluorescence loss in photobleaching (FLIP) of complete hoops containing YFP–CSCs, we demonstrate movement of the complexes beneath the nascent secondary wall in developing xylem vessels. We have devised a method for determining particle velocities for particles moving around a cylindrical object using data from FLIP. By applying this method to the hoops of YFP–CSCs of the developing vessels, we have obtained the first estimates of speed of these complexes. These speeds are calculated to be in excess of 7 μm s–1 and are far higher than those speeds previously reported for the primary wall complex. These high speeds are unlikely to be consistent with CSC movement being attributed to cellulose synthesis alone, and suggest the existence of a highly motile compartment beneath the nascent secondary wall.

Keywords: Arabidopsis; Cellulose synthase complex; FLIP; Secondary cell wall; Xylem; YFP.

Journal Article.  5874 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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