Journal Article

Arabidopsis Mutants Carrying Chimeric Sigma Factor Genes Reveal Regulatory Determinants for Plastid Gene Expression

Jennifer Schweer, Simon Geimer, Jörg Meurer and Gerhard Link

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 7, pages 1382-1386
Published in print July 2009 | ISSN: 0032-0781
Published online May 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp069
Arabidopsis Mutants Carrying Chimeric Sigma Factor Genes Reveal Regulatory Determinants for Plastid Gene Expression

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Like bacteria, plastids contain sigma factors for promoter binding and transcription initiation. Accumulating evidence suggests that members of the plant sigma factor family can have specialized non-redundant roles in terms of promoter preference in various developmental and environmental situations. To specify regulatory determinants, we have chosen pairwise exchange of portions of Arabidopsis sigma coding regions, followed by transformation of the chimeric constructs into a sigma 6 knockout line. The resulting phenotypes and plastid RNA patterns point to an important though not exclusive role for the highly variable N-terminal portion of plant sigma proteins.

Keywords: Arabidopsis; Chloroplast transcription; Chimeric sigma factors; Domain swap experiments; Regulatory determinants

Journal Article.  2610 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.