Journal Article

Differential Effects of a Transgene to Confer Low Phytic Acid in Caryopses Located at Different Positions in Rice Panicles

Mio Kuwano, Fumio Takaiwa and Kaoru T. Yoshida

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 7, pages 1387-1392
Published in print July 2009 | ISSN: 0032-0781
Published online May 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp071
Differential Effects of a Transgene to Confer Low Phytic Acid in Caryopses Located at Different Positions in Rice Panicles

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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In previous studies, we attempted to reduce phytic acid in rice seeds by silencing the 1d-myo-inositol 3-phosphate synthase gene, RINO1, using an antisense sequence under the control of the rice glutelin GluB-1 promoter. The stable transgenic line showed a weak low phytic acid phenotype. In this study, we show that the position of the caryopsis in the panicle might affect the level of gene silencing through a difference in temporal and spatial expression patterns between RINO1 and GluB-1 promoters, resulting in a large variation in Pi levels and a small increase in Pi in the transgenic seeds.

Keywords: Gene silencing; Glutelin promoter; Myo-inositol 3-phosphate synthase; Oryza sativa L.; Phytic acid; Transgenic plants

Journal Article.  2985 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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