Journal Article

Membrane-Associated, Boron-Interacting Proteins Isolated by Boronate Affinity Chromatography

Monika A. Wimmer, Günter Lochnit, Elias Bassil, Karl H. Mühling and Heiner E. Goldbach

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 7, pages 1292-1304
Published in print July 2009 | ISSN: 0032-0781
Published online May 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp073
Membrane-Associated, Boron-Interacting Proteins Isolated by Boronate Affinity Chromatography

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Boron deficiency symptoms point to a role for boron in plant membranes, but the molecular partners interacting with boron have not yet been identified. The objective of the present study was to isolate and identify membrane-associated proteins with an ability to interact with boron. Boron-interacting proteins were isolated from root microsomal preparations of arabidopsis (Arabidopsis thaliana) and maize (Zea mays) using phenylboronate affinity chromatography, subsequently separated by two-dimensional gel electrophoresis and identified using MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) peptide mass fingerprinting. Twenty-six boron-binding membrane-associated proteins were identified in A. thaliana, and nine in Z. mays roots. Additional uniden-tified proteins were also present. Common to both species were the β-subunit of mitochondrial ATP synthase, several β-glucosidases, a luminal-binding protein and fructose bisphosphate aldolase. In A. thaliana, binding of these proteins to boron was significantly reduced after 4 d of boron deprivation. The relatively high number of diverse proteins identified as boron interacting, many of which are usually enriched in membrane microdomains, supports the hypothesis that boron plays a role in plant membranes by cross-linking glycoproteins, and may be involved in their recruitment to membrane microdomains.

Keywords: Affinity chromatography; Arabidopsis thaliana; Boron; Membrane; Proteomics; Zea mays

Journal Article.  7253 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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