Journal Article

Functional Analysis of Transcription Factors in Arabidopsis

Nobutaka Mitsuda and Masaru Ohme-Takagi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 7, pages 1232-1248
Published in print July 2009 | ISSN: 0032-0781
Published online May 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp075
Functional Analysis of Transcription Factors in Arabidopsis

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  • Biochemistry
  • Molecular and Cell Biology
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Transcription factors (TFs) regulate the expression of genes at the transcriptional level. Modification of TF activity dynamically alters the transcriptome, which leads to metabolic and phenotypic changes. Thus, functional analysis of TFs using ‘omics-based’ methodologies is one of the most important areas of the post-genome era. In this mini-review, we present an overview of Arabidopsis TFs and introduce strategies for the functional analysis of plant TFs, which include both traditional and recently developed technologies. These strategies can be assigned to five categories: bioinformatic analysis; analysis of molecular function; expression analysis; phenotype analysis; and network analysis for the description of entire transcriptional regulatory networks.

Keywords: Arabidopsis; Bioinformatics; Functional analysis; Methodology; Transcription factor

Journal Article.  9195 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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