Journal Article

Activation of Sucrose Transport in Defoliated <i>Lolium perenne</i> L.: An Example of Apoplastic Phloem Loading Plasticity

Alexandre Berthier, Marie Desclos, Véronique Amiard, Annette Morvan-Bertrand, Barbara Demmig-Adams, William W. Adams, Robert Turgeon, Marie-Pascale Prud’homme and Nathalie Noiraud-Romy

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 7, pages 1329-1344
Published in print July 2009 | ISSN: 0032-0781
Published online June 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp081
Activation of Sucrose Transport in Defoliated Lolium perenne L.: An Example of Apoplastic Phloem Loading Plasticity

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The pathway of carbon phloem loading was examined in leaf tissues of the forage grass Lolium perenne. The effect of defoliation (leaf blade removal) on sucrose transport capacity was assessed in leaf sheaths as the major carbon source for regrowth. The pathway of carbon transport was assessed via a combination of electron microscopy, plasmolysis experiments and plasma membrane vesicles (PMVs) purified by aqueous two-phase partitioning from the microsomal fraction. Results support an apoplastic phloem loading mechanism. Imposition of an artificial proton-motive force to PMVs from leaf sheaths energized an active, transient and saturable uptake of sucrose (Suc). The affinity of Suc carriers for Suc was 580 μM in leaf sheaths of undefoliated plants. Defoliation induced a decrease of Km followed by an increase of Vmax. A transporter was isolated from stubble (including leaf sheaths) cDNA libraries and functionally expressed in yeast. The level of L.perenne SUcrose Transporter 1 (LpSUT1) expression increased in leaf sheaths in response to defoliation. Taken together, the results indicate that Suc transport capacity increased in leaf sheaths of L. perenne in response to leaf blade removal. This increase might imply de novo synthesis of Suc transporters, including LpSUT1, and may represent one of the mechanisms contributing to rapid refoliation.

Keywords: Defoliation; Lolium perenne; Sucrose transport

Journal Article.  8338 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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