Journal Article

Identification of <i>cis-</i>Localization Elements that Target Glutelin RNAs to a Specific Subdomain of the Cortical Endoplasmic Reticulum in Rice Endosperm Cells

Haruhiko Washida, Sachiyo Kaneko, Naoko Crofts, Aya Sugino, Changlin Wang and Thomas W Okita

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 9, pages 1710-1714
Published in print September 2009 | ISSN: 0032-0781
Published online July 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp103
Identification of cis-Localization Elements that Target Glutelin RNAs to a Specific Subdomain of the Cortical Endoplasmic Reticulum in Rice Endosperm Cells

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  • Biochemistry
  • Molecular and Cell Biology
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Rice glutelin RNAs are localized to the cisternal endoplasmic reticulum (ER) by a regulated RNA transport process requiring specific cis-localization elements. We set out to identify these glutelin sequences by their dominant character of being able to re-direct the normal protein body ER localization of a maize 10 kDa δ-zein RNA to the cisternal ER. In situ RNA localization analysis showed that the glutelin RNA contains multiple cis-localization elements; two located at the 5′ and 3′ ends of the coding sequences and a third located within the 3′-untranslated region. These three regions contain two conserved sequences, suggesting that these RNA recognition signals may be sequence based.

Keywords: Cis-localization element; Endoplasmic reticulum; Glutelin; In situ RT-PCR; Rice; RNA targeting

Journal Article.  2053 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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