Journal Article

A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2′-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants

Yusuke Kakei, Isomaro Yamaguchi, Takanori Kobayashi, Michiko Takahashi, Hiromi Nakanishi, Takashi Yamakawa and Naoko K. Nishizawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 11, pages 1988-1993
Published in print November 2009 | ISSN: 0032-0781
Published online October 2009 | e-ISSN: 1471-9053 | DOI:

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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A highly sensitive quantitative method for assaying nicotianamine (NA) and 2′-deoxymugineic acid (DMA) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) was developed. The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmethoxycarbonyl chloride. The amounts of NA and DMA in 10 μl of xylem sap from rice cultivated under iron (Fe)-sufficient and Fe-deficient conditions were quantified without concentration. In Fe-sufficient plants, the concentrations of NA and DMA were almost equal to that of Fe. In Fe-deficient plants, the concentration of NA did not change significantly, whereas that of DMA increased markedly.

Keywords: 2′-Deoxymugineic acid; Fe deficiency; Nicotianamine; Xylem transport

Journal Article.  2938 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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