Journal Article

Dynamic Aspects of Ion Accumulation by Vesicle Traffic Under Salt Stress in Arabidopsis

Kohei Hamaji, Megumi Nagira, Katsuhisa Yoshida, Miwa Ohnishi, Yoshihisa Oda, Tomohiro Uemura, Tatsuaki Goh, Masa H. Sato, Miyo T. Morita, Masao Tasaka, Sei-ichiro Hasezawa, Akihiko Nakano, Ikuko Hara-Nishimura, Masayoshi Maeshima, Hidehiro Fukaki and Tetsuro Mimura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 12, pages 2023-2033
Published in print December 2009 | ISSN: 0032-0781
Published online September 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp143
Dynamic Aspects of Ion Accumulation by Vesicle Traffic Under Salt Stress in Arabidopsis

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

The intracellular membrane dynamics of Arabidopsis cells under high salt treatment were investigated. When Arabidopsis was treated with high levels of NaCl in hydroponic culture, root tip cells showed rapid changes in the vacuolar volume, a decrease in the number of small acid compartments, active movement of vesicles and accumulation of Na+ both in the central vacuole and in the vesicles around the main vacuole observed with the Na+-dependent fluorescence of Sodium Green. Detailed observation of Arabidopsis suspension-cultured cells under high salt treatment showed a similar pattern of response to that observed in root tip cells. Immunostaining of suspension-cultured cells with antibodies against AtNHX1 clearly showed the occurrence of dotted fluorescence in the cytoplasm only under salt treatment. We also confirmed the existence of AtNHX1 in the vacuolar membrane isolated from suspension-cultured cells with immunofluorescence. Knockout of the vacuolar Qa-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) protein VAM3/SYP22 caused an increase in salt tolerance. In mutant plants, the distribution of Na+ between roots and shoots differed from that of wild-type plants, with Na+ accumulating more in roots and less in the shoots of the mutant plants. The role of vesicle traffic under salt stress is discussed.

Keywords: Arabidopsis suspension-cultured cells • Na+/H+ antiporter • NHX • Salt tolerance • SNARE • Vacuole

Journal Article.  4821 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.