Journal Article

Mechanisms of Progressive Water Deficit Tolerance and Growth Recovery of Chinese Maize Foundation Genotypes Huangzao 4 and Chang 7-2, Which are Proposed on the Basis of Comparison of Physiological and Transcriptomic Responses

Youzhi Li, Changbin Sun, Zhangbao Huang, Jianlong Pan, Liang Wang and Xianwei Fan

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 12, pages 2092-2111
Published in print December 2009 | ISSN: 0032-0781
Published online September 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp145
Mechanisms of Progressive Water Deficit Tolerance and Growth Recovery of Chinese Maize Foundation Genotypes Huangzao 4 and Chang 7-2, Which are Proposed on the Basis of Comparison of Physiological and Transcriptomic Responses

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

The maize inbred lines Huangzao 4 (HZ4) and Chang 7-2 (C7-2) are the foundation genotypes key to maize cross-breeding in China. C7-2 is derived from HZ-4. In this study, changes in phenotype, physiology and gene expression of three-leaf-old seedlings of HZ4 and C7-2 under the conditions of progressive water deficit (WD) and re-watering (RW) were compared to gain knowledge for breeding new maize foundation genotypes with higher drought tolerance. Progressive WD was produced by adding polyethylene glycol (PEG 6000) at 24 h intervals (24, 48 and 72 h) in Hoagland’s nutrient solution, resulting in water potentials of −0.15, −0.3 and −0.5 MPa. The seedlings treated for 24 h at −0.3 MPa were subjected to RW in the solution without complementation with PEG. The results showed that C7-2 seedlings are more tolerant to progressive WD than HZ4 seedlings in part because the former have a larger stomatal resistance, a relatively stronger leaf water-holding capacity, and a timely and stable increase in activities of antioxidant enzymes (superoxide dismutase and peroxidase) especially in roots upon WD. Oligonucleotide probe array-based analysis uncovered a number of WD- and RW-regulated genes in both inbred lines, and clearly indicated that fine transcriptional coordination between maize leaves and roots is one of the factors constituting higher WD tolerance and a greater ability for growth recovery from WD. On the basis of the resulting data and co-regulation of responsive genes in tissues, we propose a model for the whole maize plant tolerance to growth and recovery from WD.

Keywords: Foundation genotype • Maize • Physiology • Re-watering • Transcription • Water deficit

Journal Article.  7352 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.