Journal Article

Overexpression of a <i>Brassica rapa NGATHA</i> Gene in <i>Arabidopsis thaliana</i> Negatively Affects Cell Proliferation During Lateral Organ and Root Growth

So Hyun Kwon, Byung Ha Lee, Eun Yu Kim, Young Sam Seo, Sangman Lee, Woo Taek Kim, Jong Tae Song and Jeong Hoe Kim

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 12, pages 2162-2173
Published in print December 2009 | ISSN: 0032-0781
Published online September 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp150
Overexpression of a Brassica rapa NGATHA Gene in Arabidopsis thaliana Negatively Affects Cell Proliferation During Lateral Organ and Root Growth

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In an effort to elucidate biological functions of transcription factors of Brassica rapa L. (ssp. pekinensis), an NGATHA homolog, BrNGA1, that belongs to the B3-type transcription factor superfamily was identified and expressed in Arabidopsis thaliana under the control of the cauliflower mosaic virus (CaMV) 35S promoter. Arabidopsis plants overexpressing BrNGA1, named BrNGA1ox, displayed markedly reduced organ growth compared with the wild type: lateral organs, such as leaves, flowers and cotyledons, were small and distinctively narrow, and their root growth was also severely retarded. Reduced sizes of BrNGA1ox organs were mainly due to reduction in cell numbers. Kinematic analysis of leaf growth revealed that both the rate and duration of cell proliferation declined during organogenesis, which was consistent with the reduced expression of cyclin genes. Reduction in organ growth was strongly correlated with the small size of meristematic cell pools in the shoot and root meristems. Taken together, these data indicate that BrNGA1 acts as a negative regulator of cell proliferation and may do so, in part, by regulating the size of the meristematic cell pool.

Keywords: Arabidopsis • B3 transcription factors • Brassica rapa • NGATHA • Organ size and shape

Journal Article.  6629 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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