Journal Article

Identification of Zinc-Responsive Proteins in the Roots of <i>Arabidopsis thaliana</i> Using a Highly Improved Method of Two-Dimensional Electrophoresis

Yoichiro Fukao, Ali Ferjani, Masayuki Fujiwara, Yuka Nishimori and Iwao Ohtsu

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 50, issue 12, pages 2234-2239
Published in print December 2009 | ISSN: 0032-0781
Published online September 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp154
Identification of Zinc-Responsive Proteins in the Roots of Arabidopsis thaliana Using a Highly Improved Method of Two-Dimensional Electrophoresis

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Zinc (Zn) is an essential micronutrient for various physiological and metabolic processes in plants, although it is toxic in excess. To understand better Zn-responsive proteins, we developed a highly improved method of isoelectric focusing (IEF) in which whole lysate from Arabidopsis roots is subjected to IEF without any desalting steps. In this method, samples extracted with lysis buffer containing 1.5% SDS can also be directly applied to IEF. By applying this method to Zn-treated Arabidopsis roots, 10 up-regulated and 17 down-regulated proteins were identified, 15 of which showed a significant correlation with previously reported transcriptomic data.

Keywords: Arabidopsis thaliana • 2-D gel electrophoresis • IEF • Microsomal fraction • SDS • Zn stress

Journal Article.  3307 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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