Journal Article

Knock-down of <i>OsDCL2</i> in Rice Negatively Affects Maintenance of the Endogenous dsRNA Virus, <i>Oryza sativa</i> Endornavirus

Syunichi Urayama, Hiromitsu Moriyama, Nanako Aoki, Yukihiro Nakazawa, Ryo Okada, Eri Kiyota, Daisuke Miki, Ko Shimamoto and Toshiyuki Fukuhara

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 1, pages 58-67
Published in print January 2010 | ISSN: 0032-0781
Published online November 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp167
Knock-down of OsDCL2 in Rice Negatively Affects Maintenance of the Endogenous dsRNA Virus, Oryza sativa Endornavirus

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An endogenous double-stranded RNA (dsRNA), which has recently been recognized as the dsRNA virus Oryza sativa endornavirus (OsEV), is found in many strains of cultivated rice (Oryza sativa). Small RNAs derived from OsEV dsRNA were detected, indicating that the RNA silencing machinery recognizes OsEV dsRNA. The existence of OsEV in knock-down (KD) lines of five genes of RNA-dependent RNA polymerase (OsRDR1–OsRDR5) or two genes of Dicer-like protein (OsDCL2 or OsDCL3a) was examined to characterize the relationship between the host RNA silencing system and the propagation of this dsRNA virus. OsEV was not detected in OsRDR4-KD or OsDCL2-KD T1 lines. We attempted to introduce OsEV into these KD lines by crossing them with OsEV-carrying plants because of the efficient transmission of OsEV to F1 plants via pollen or ova. All OsRDR4-KD but only some OsDCL2-KD F1 plants contained OsEV. Some OsDCL2-KD F1 plants consisted of OsEV-carrying and OsEV-free cells. These results suggest that the maintenance of OsEV is unstable in OsDCL2-KD plants. Furthermore, the amount of OsEV-derived small interfering RNA (vsiRNA) in the OsDCL2-KD plants increased relative to the wild type. This increased level of vsiRNA may cause OsEV instability during cell division.

Keywords: dsRNA; Endornavirus; Knock-down; OsDCL2; Rice; RNA silencing

Journal Article.  6125 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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