Journal Article

The <i>HSP</i> Terminator of <i>Arabidopsis thaliana</i> Increases Gene Expression in Plant Cells

Shingo Nagaya, Kazue Kawamura, Atsuhiko Shinmyo and Ko Kato

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 2, pages 328-332
Published in print February 2010 | ISSN: 0032-0781
Published online December 2009 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcp188
The HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant Cells

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  • Biochemistry
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To express a foreign gene in plants effectively, a good expression system is required. Here we describe the identification of a transcriptional terminator that supports increased levels of expression. The terminators of several Arabidopsis genes were examined in transfected Arabidopsis T87 protoplasts. The heat shock protein 18.2 (HSP) terminator was the most effective in supporting increased levels of expression. The HSP terminator increases mRNA levels of both transiently and stably expressed transgenes approximately 2-fold more than the NOS (nopaline synthase) terminator. When combined with the HSP terminator, a translational enhancer increased gene expression levels approximately 60- to 100-fold in transgenic plants.

Keywords: Arabidopsis thaliana; 3′ End region; Gene expression; Heat shock protein gene; Polyadenylation; Terminator

Journal Article.  2553 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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