Journal Article

The PsbK Subunit is Required for the Stable Assembly and Stability of Other Small Subunits in the PSII complex in the Thermophilic Cyanobacterium <i>Thermosynechococcus elongatus</i> BP-1

Masako Iwai, Takehiro Suzuki, Akiko Kamiyama, Isamu Sakurai, Naoshi Dohmae, Yasunori Inoue and Masahiko Ikeuchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 4, pages 554-560
Published in print April 2010 | ISSN: 0032-0781
Published online April 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq020
The PsbK Subunit is Required for the Stable Assembly and Stability of Other Small Subunits in the PSII complex in the Thermophilic Cyanobacterium Thermosynechococcus elongatus BP-1

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PsbK is a small membrane protein of the PSII core complex and is highly conserved from cyanobacteria to plants. Here, we studied its role in the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1, by focusing on a psbK disruptant with hexahistidine-tagged CP47. The psbK disruptant showed photoautotrophic growth comparable with that of the wild type under a wide range of light conditions. The mutant PSII complex retained the oxygen-evolving activity with a unique modification of the acceptor QB site. N-terminal sequencing revealed that Ycf12 and PsbZ proteins were lost in the PSII complex prepared from the mutant. Immunoblotting detected reduced accumulation of PsbZ in the mutant thylakoid. These results suggest that PsbK is required not only for association of PsbZ and Ycf12 with the isolated PSII complex but also for the stabilization of PsbZ in the thylakoid membrane.

Keywords: PSII; Psb30; PsbK; PsbZ; Thermosynechococcus elongatus; Ycf12

Journal Article.  4042 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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