Journal Article

Cytoplasmic–Nuclear Genomic Barriers in Rice Pollen Development Revealed by Comparison of Global Gene Expression Profiles among Five Independent Cytoplasmic Male Sterile Lines

Sota Fujii, Mari Yamada, Masahiro Fujita, Etsuko Itabashi, Kazuki Hamada, Kentaro Yano, Nori Kurata and Kinya Toriyama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 4, pages 610-620
Published in print April 2010 | ISSN: 0032-0781
Published online April 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq026
Cytoplasmic–Nuclear Genomic Barriers in Rice Pollen Development Revealed by Comparison of Global Gene Expression Profiles among Five Independent Cytoplasmic Male Sterile Lines

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Cytoplasmic male sterility (CMS) is one of the most ideal phenomena known in higher plants to describe the incompatibilities between mitochondrial–nuclear genomic interactions. To elucidate the dependency of pollen development on mitochondrial genotypes and cytoplasmic–nuclear genomic barriers, we employed five CMS isogenic lines of rice, CW-, W11-, LD-, BT- and WA-type CMS lines, that exhibit distinct pollen-defective phenotypes, and we characterized the CMS phenotypes and the nuclear gene expression patterns in conjunction with their mitochondrial genomic structures. These five CMS lines carried independent mitotypes, and W11, LD and BT mitochondrial genomes were relatively close with respect to their phylogeny. In anthers at the uninucleate microspore and bicellular pollen stages, 8,199 genes significantly changed their expression in at least one of the CMS lines. Common expression patterns were observed in BT, LD and W11 after k-means clustering. Among the genes encoding putative mitochondrial proteins, ALTERNATIVE OXIDASE 1A, a gene for the well-known mitochondrial stress marker, was included in the group ectopically up-regulated in anthers at the bicellular pollen stage of BT, LD and W11. Several other clusters were also regulated in a cytoplasm-specific manner during pollen development. These clear similarities in gene regulatory networks of BT-, LD- and W11-CMS lines indicate that the phylogenetic relationships of the mitochondrial genotypes are strongly correlated with nuclear gene expression patterns and pollen abortion phenotypes, providing evidence of the mitochondrial epistacy over the nuclear genome during pollen development.

Keywords: Cytoplasmic male sterility; Epistacy; Microarray; Mitochondria; Oryza sativa L; Pollen development

Journal Article.  7779 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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