Journal Article

Mutations in the Arabidopsis Nuclear-Encoded Mitochondrial Phage-Type RNA Polymerase Gene <i>RPOTm</i> Led to Defects in Pollen Tube Growth, Female Gametogenesis and Embryogenesis

Xiao-Yun Tan, Xiao-Lei Liu, Wei Wang, Dong-Jie Jia, Li-Qun Chen, Xue-Qin Zhang and De Ye

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 4, pages 635-649
Published in print April 2010 | ISSN: 0032-0781
Published online April 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq029
Mutations in the Arabidopsis Nuclear-Encoded Mitochondrial Phage-Type RNA Polymerase Gene RPOTm Led to Defects in Pollen Tube Growth, Female Gametogenesis and Embryogenesis

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

The mitochondrial genes in Arabidopsis thaliana are transcribed by a small family of nuclear-encoded T3/T7 phage-type RNA polymerases (RPOTs). At least two nuclear-encoded RPOTs (RPOTm and RPOTmp) are located in mitochondria in A. thaliana. Their genetic roles are largely unknown. Here we report the characterization of novel mutations in the A. thaliana RPOTm gene. The mutations did not affect pollen formation, but significantly retarded the growth of the rpoTm mutant pollen tubes and had an impact on the fusion of the polar nuclei in the rpoTm mutant embryo sacs. Moreover, development of the rpoTm/– mutant embryo was arrested at the globular stage. The rpoTm rpoTmp double mutation could enhance the rpoTm mutant phenotype. Expression of RPOTmp under control of the RPOTm promoter could not complement the phenotype of the rpoTm mutations. All these data indicate that RPOTm is important for normal pollen tube growth, female gametogenesis and embryo development, and has distinct genetic and molecular roles in plant development, which cannot be replaced by RPOTmp.

Keywords: Arabidopsis; Embryogenesis; Gametogenesis; Mitochondria; Phage-type RNA polymerase; Pollen

Journal Article.  7516 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.