Journal Article

Overexpressing a Putative Aquaporin Gene from Wheat, <i>TaNIP</i>, Enhances Salt Tolerance in Transgenic Arabidopsis

Zhenxian Gao, Xiaoliang He, Baocun Zhao, Chunjiang Zhou, Yingzhu Liang, Rongchao Ge, Yinzhu Shen and Zhanjing Huang

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 5, pages 767-775
Published in print May 2010 | ISSN: 0032-0781
Published online March 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq036
Overexpressing a Putative Aquaporin Gene from Wheat, TaNIP, Enhances Salt Tolerance in Transgenic Arabidopsis

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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High soil salinity is a major abiotic stress in plant agriculture worldwide. Here, we report the characterization of a novel aquaporin gene TaNIP (Triticum asetivum L. nodulin 26-like intrinsic protein), which was involved in salt tolerance pathways in plants. TaNIP was identified and cloned through the gene chip expression analysis of a salt-tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription–PCR (Q-RT–PCR) was used to detect TaNIP expression under salt, drought, cold and ABA treatment. The overexpression of TaNIP in transgenic Arabidopsis produced higher salt tolerance than wild-type plants. Localization analysis showed that TaNIP proteins tagged with green fluorescent protein (GFP) were localized to the cell plasma membrane. Under salt stress treatment, TaNIP-overexpressing Arabidopsis accumulated higher K+, Ca2+ and proline contents and lower Na+ level than the wild-type plants. The overexpression of TaNIP in transgenic Arabidopsis also up-regulated the expression of a number of stress-associated genes. Our results suggest that TaNIP plays an important role in salt tolerance in Arabidopsis and can also enhance plants’ tolerance to other abiotic stresses.

Keywords: Aquaporin; Salt tolerance; TaNIP; Wheat

Journal Article.  4167 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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