Journal Article

Simultaneous Determination of In Vivo Plastoquinone and Ubiquinone Redox States by HPLC-Based Analysis

Keisuke Yoshida, Masaru Shibata, Ichiro Terashima and Ko Noguchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 5, pages 836-841
Published in print May 2010 | ISSN: 0032-0781
Published online April 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq044
Simultaneous Determination of In Vivo Plastoquinone and Ubiquinone Redox States by HPLC-Based Analysis

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For a better understanding of the metabolic interaction between chloroplasts and mitochondria, it is important to analyze the in vivo redox states of the electron transport chains in both organelles at the same time. For this purpose, we devised an HPLC-based measurement system simultaneously analyzing plastoquinone (PQ) and ubiquinone (UQ) contents and redox states. Using this system, we discovered that, in addition to PQ, the reduction levels of UQ were elevated under high-light conditions. We also provide direct evidence that mitochondrial alternative oxidase contributes to alleviate UQ over-reduction under such conditions.

Keywords: Alternative oxidase; Arabidopsis thaliana; Electron transport chain; HPLC; Plastoquinone; Ubiquinone

Journal Article.  3136 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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