Journal Article

Deregulated Chlorophyll <i>b</i> Synthesis Reduces the Energy Transfer Rate Between Photosynthetic Pigments and Induces Photodamage in <i>Arabidopsis thaliana</i>

Yasuhito Sakuraba, Makio Yokono, Seiji Akimoto, Ryouichi Tanaka and Ayumi Tanaka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 6, pages 1055-1065
Published in print June 2010 | ISSN: 0032-0781
Published online April 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq050
Deregulated Chlorophyll b Synthesis Reduces the Energy Transfer Rate Between Photosynthetic Pigments and Induces Photodamage in Arabidopsis thaliana

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Chl b is one of the major light-harvesting pigments in land plants. The synthesis of Chl b is strictly regulated in response to light conditions in order to control the antenna size of photosystems. Regulation of Chl b also affects its distribution as it occurs preferentially in the peripheral antenna complexes. However, it has not been experimentally shown how plants respond to environmental conditions when they accumulate excess Chl b. Previously, we produced an Arabidopsis transgenic plant (referred to as the BC plant) in which Chl b biosynthesis was enhanced. In this study, we analyzed the photosynthetic properties and genome-wide gene expression in this plant under high light conditions in order to understand the effects of deregulated Chl b biosynthesis. The energy transfer rates between Chl a molecules in PSII decreased and H2O2 accumulated extensively in the BC plant. Microarray analysis revealed that a group of genes involved in anthocyanin biosynthesis was down-regulated and that another group of genes, reported to be sensitive to H2O2, was up-regulated in the BC plant. We also found that anthocyanin levels were low, which was consistent with the results of the microarray analysis. These results indicate that deregulation of Chl b caused severe photodamage and altered gene expression profiles under strong illumination. The importance of the regulation of Chl b synthesis is discussed in relation to the correct localization of Chl b and gene expression.

Keywords: Chlorophyll b; Chlorophyllide a oxygenase; Photooxidative damage; Reactive oxygen species (ROS)

Journal Article.  5756 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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