Journal Article

Physiological Impact of Intrinsic ADP Inhibition of Cyanobacterial F<sub>o</sub>F<sub>1</sub> Conferred by the Inherent Sequence Inserted into the γ Subunit

Ei-Ichiro Sunamura, Hiroki Konno, Mari Imashimizu-Kobayashi, Yasushi Sugano and Toru Hisabori

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 6, pages 855-865
Published in print June 2010 | ISSN: 0032-0781
Published online April 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq061
Physiological Impact of Intrinsic ADP Inhibition of Cyanobacterial FoF1 Conferred by the Inherent Sequence Inserted into the γ Subunit

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The FoF1-ATPase, which synthesizes ATP with a rotary motion, is highly regulated in vivo in order to function efficiently, although there remains a limited understanding of the physiological significance of this regulation. Compared with its bacterial and mitochondrial counterparts, the γ subunit of cyanobacterial F1, which makes up the central shaft of the motor enzyme, contains an additional inserted region. Although deletion of this region results in the acceleration of the rate of ATP hydrolysis, the functional significance of the region has not yet been determined. By analysis of rotation, we successfully determined that this region confers the ability to shift frequently into an ADP inhibition state; this is a highly conserved regulatory mechanism which prevents ATP synthase from carrying out the reverse reaction. We believe that the physiological significance of this increased likelihood of shifting into the ADP inhibition state allows the intracellular ATP levels to be maintained, which is especially critical for photosynthetic organisms.

Keywords: ADP inhibition; ATP synthase; Cyanobacteria; γ subunit; Regulation

Journal Article.  7040 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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