Journal Article

Molecular Cloning and Characterization of a Broad Substrate Terpenoid Oxidoreductase from <i>Artemisia annua</i>

Anna-Margareta Rydén, Carolien Ruyter-Spira, Ralph Litjens, Shunji Takahashi, Wim Quax, Hiroyuki Osada, Harro Bouwmeester and Oliver Kayser

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 7, pages 1219-1228
Published in print July 2010 | ISSN: 0032-0781
Published online May 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq073
Molecular Cloning and Characterization of a Broad Substrate Terpenoid Oxidoreductase from Artemisia annua

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From Artemisia annua L., a new oxidoreductase (Red 1) was cloned, sequenced and functionally characterized. Through bioinformatics, heterologous protein expression and enzyme substrate conversion assays, the elucidation of the enzymatic capacities of Red1 was achieved. Red1 acts on monoterpenoids, and in particular functions as a menthone:neomenthol oxidoreductase. The kinetic parameter kcat/Km was determined to be 939-fold more efficient for the reduction of (−)-menthone to (+)-neomenthol than results previously reported for the menthone:neomenthol reductase from Mentha × piperita. Based on its kinetic properties, the possible use of Red1 in biological crop protection is discussed.

Keywords: Artemisia annua L; Menthone; Oxidoreductase; red1; Terpenoids

Journal Article.  4083 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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