Journal Article

Copper Metallochaperones are Required for the Assembly of Bacteroid Cytochrome <i>c</i> Oxidase Which is Functioning for Nitrogen Fixation in Soybean Nodules

Hatthaya Arunothayanan, Mika Nomura, Rie Hamaguchi, Manabu Itakura, Kiwamu Minamisawa and Shigeyuki Tajima

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 7, pages 1242-1246
Published in print July 2010 | ISSN: 0032-0781
Published online June 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq079
Copper Metallochaperones are Required for the Assembly of Bacteroid Cytochrome c Oxidase Which is Functioning for Nitrogen Fixation in Soybean Nodules

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Bradyrhizobium japonicum, a symbiotic nitrogen-fixing bacterium for Glycine max, has complex respiratory electron transport chains. Bll4880 contained a copper-binding motif for metallochaperone, H(M)X10MX21HXM. A mutant strain, Bj4880, induced nodules with lower acetylene reduction activity. A double mutant, Bj4880-1131, which had inserted mutations both in blr1131, a gene of the Sco1-like protein, and in bll4880, induced nodules of significant Fix phenotype and low cytochrome c oxidase (Cco) activity in the bacteroid. Our data suggest that bll4880 protein is involved in copper ion delivery to Cco through blr1131 protein, and the expression of both proteins was induced under microaerobic conditions.

Keywords: Bradyrhizobium japonicum; Cytochrome c oxidase; Metallochaperone

Journal Article.  2405 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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