Journal Article

Overexpression of a Profilin (GhPFN2) Promotes the Progression of Developmental Phases in Cotton Fibers

Juan Wang, Hai-Yun Wang, Pi-Ming Zhao, Li-Bo Han, Gai-Li Jiao, Yi-Yan Zheng, Shan-Jin Huang and Gui-Xian Xia

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 8, pages 1276-1290
Published in print August 2010 | ISSN: 0032-0781
Published online June 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq086
Overexpression of a Profilin (GhPFN2) Promotes the Progression of Developmental Phases in Cotton Fibers

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  • Biochemistry
  • Molecular and Cell Biology
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Cotton fiber development at the stages of elongation and secondary wall synthesis determines the traits of fiber length and strength. To date, the mechanisms controlling the progression of these two phases remain elusive. In this work, the function of a fiber-preferential actin-binding protein (GhPFN2) was characterized by cytological and molecular studies on the fibers of transgenic green-colored cotton (Gossypium hirsutum) through three successive generations. Overexpression of GhPFN2 caused pre-terminated cell elongation, resulting in a marked decrease in the length of mature fibers. Cytoskeleton staining and quantitative assay revealed that thicker and more abundant F-actin bundles formed during the elongation stage in GhPFN2-overexpressing fibers. Accompanying this alteration, the developmental reorientation of transverse microtubules to the oblique direction was advanced by 2 d at the period of transition from elongation to secondary wall deposition. Birefringence and reverse transcription–PCR analyses showed that earlier onset of secondary wall synthesis occurred in parallel. These data demonstrate that formation of the higher actin structure plays a determinant role in the progression of developmental phases in cotton fibers, and that GhPFN2 acts as a critical modulator in this process. Such a function of the actin cytoskeleton in cell phase conversion may be common to other secondary wall-containing plant cells.

Keywords: Actin cytoskeleton; Cotton fiber; Gossypium hirsutum; Microtubule; Profilin; Secondary wall deposition

Journal Article.  8591 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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