Journal Article

<i>plenty</i>, a Novel Hypernodulation Mutant in <i>Lotus japonicus</i>

Chie Yoshida, Sachiko Funayama-Noguchi and Masayoshi Kawaguchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 9, pages 1425-1435
Published in print September 2010 | ISSN: 0032-0781
Published online August 2010 | e-ISSN: 1471-9053 | DOI:
plenty, a Novel Hypernodulation Mutant in Lotus japonicus

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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Nitrogen fixation in nodules that contain symbiotic rhizobial bacteria enables legumes to thrive in nitrogen-poor soils. However, this symbiosis is energy consuming. Therefore, legumes strictly control nodulation at both local and systemic levels. Mutants deficient in such controls exhibit a range of phenotypes from non-nodulation to hypernodulation. Here, we isolated a novel hypernodulation mutant from the M2 progeny derived from Lotus japonicus MG-20 seeds mutagenized by irradiation with a carbon ion beam. We named the mutant 'plenty' because it formed more nodules than the wild-type MG-20. The nodulation zone in the plenty mutant was wider than that in the wild type, but not as enhanced as those in other previously reported hypernodulation mutants such as har1, klv or tml of L. japonicus. Unlike these hypernodulation mutants, the plenty mutant developed nodules of the same size as MG-20. Overall, the plenty mutant exhibited a unique phenotype of moderate hypernodulation. However, a biomass assay indicated that this unique pattern of hypernodulation was a hindrance to host plant growth. The plenty mutant displayed some tolerance to external nitrates and a normal triple response to ethylene. Grafting experiments demonstrated that the root of plenty was responsible for its hypernodulation phenotype. Genetic mapping indicated that the PLENTY gene was located on chromosome 2.

Keywords: Biomass; Ion beam irradiation; Legume–Rhizobium symbiosis; Lotus japonicus; Nitrate; Root-determined hypernodulation

Journal Article.  6130 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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