Journal Article

Microbial Volatile Emissions Promote Accumulation of Exceptionally High Levels of Starch in Leaves in Mono- and Dicotyledonous Plants

Ignacio Ezquer, Jun Li, Miroslav Ovecka, Edurne Baroja-Fernández, Francisco José Muñoz, Manuel Montero, Jessica Díaz de Cerio, Maite Hidalgo, María Teresa Sesma, Abdellatif Bahaji, Ed Etxeberria and Javier Pozueta-Romero

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 10, pages 1674-1693
Published in print October 2010 | ISSN: 0032-0781
Published online August 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq126
Microbial Volatile Emissions Promote Accumulation of Exceptionally High Levels of Starch in Leaves in Mono- and Dicotyledonous Plants

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  • Biochemistry
  • Molecular and Cell Biology
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Microbes emit volatile compounds that affect plant growth and development. However, little or nothing is known about how microbial emissions may affect primary carbohydrate metabolism in plants. In this work we explored the effect on leaf starch metabolism of volatiles released from different microbial species ranging from Gram-negative and Gram-positive bacteria to fungi. Surprisingly, we found that all microbial species tested (including plant pathogens and species not normally interacting with plants) emitted volatiles that strongly promoted starch accumulation in leaves of both mono- and dicotyledonous plants. Starch content in leaves of plants treated for 2 d with microbial volatiles was comparable with or even higher than that of reserve organs such as potato tubers. Transcriptome and enzyme activity analyses of potato leaves exposed to volatiles emitted by Alternaria alternata revealed that starch overaccumulation was accompanied by up-regulation of sucrose synthase, invertase inhibitors, starch synthase class III and IV, starch branching enzyme and glucose-6-phosphate transporter. This phenomenon, designated as MIVOISAP (microbial volatiles-induced starch accumulation process), was also accompanied by down-regulation of acid invertase, plastidial thioredoxins, starch breakdown enzymes, proteins involved in internal amino acid provision and less well defined mechanisms involving a bacterial- type stringent response. Treatment of potato leaves with fungal volatiles also resulted in enhanced levels of sucrose, ADPglucose, UDPglucose and 3-phosphoglycerate. MIVOISAP is independent of the presence of sucrose in the culture medium and is strongly repressed by cysteine supplementation. The discovery that microbial volatiles trigger starch accumulation enhancement in leaves constitutes an unreported mechanism for the elicidation of plant carbohydrate metabolism by microbes.

Keywords: Carbohydrate metabolism

Journal Article.  11214 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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