Journal Article

Cytosolic Alkalization and Cytosolic Calcium Oscillation in Arabidopsis Guard Cells Response to ABA and MeJA

Mohammad Mahbub Islam, Mohammad Anowar Hossain, Rayhanur Jannat, Shintaro Munemasa, Yoshimasa Nakamura, Izumi C. Mori and Yoshiyuki Murata

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 10, pages 1721-1730
Published in print October 2010 | ISSN: 0032-0781
Published online August 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq131
Cytosolic Alkalization and Cytosolic Calcium Oscillation in Arabidopsis Guard Cells Response to ABA and MeJA

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  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure are accompanied by cytosolic alkalization in guard cells. However, it remains to be clarified how the alkalization functions in not only ABA signaling but also MeJA. We investigated cytosolic alkalization in guard cells during ABA-, MeJA- and Ca2+-induced stomatal closure of wild type, abi1-1, abi2-1, ost1-2 and coi1 using a pH-sensitive fluorescent dye, BCECF-AM. ABA induced cytosolic alkalization in guard cells of wild-type and coi1 but not in ost1-2 guard cells whereas MeJA elicited cytosolic alkalization in wild-type and ost1-2 guard cells but not in coi1. Neither ABA nor MeJA induced cytosolic alkalization in abi1-1 and abi2-1 guard cells. Exogenous Ca2+ induced stomatal closure accompanied by cytosolic alkalization in guard cells of wild-type, abi1-1, abi2-1, ost1-2 and coi1 plants. An agent to acidify cytosol, butyrate, suppressed Ca2+-induced cytosolic alkalization and ABA-, MeJA- and Ca2+-induced cytosolic Ca2+ oscillation in wild-type guard cells to prevent stomatal closure. These results indicate that cytosolic alkalization and cytosolic Ca2+ oscillation coordinately function in ABA and MeJA signaling in Arabidopsis guard cells.

Keywords: Abscisic acid; Arabidopsis thaliana; Cytosolic calcium; Cytosolic pH; Guard cells; Methyl jasmonate

Journal Article.  5152 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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