Journal Article

Cloning and Characterization of a New Polyol Transporter (HbPLT2) in <i>Hevea brasiliensis</i>

Anaïs Dusotoit-Coucaud, Benoit Porcheron, Nicole Brunel, Panida Kongsawadworakul, Jérôme Franchel, Unshira Viboonjun, Hervé Chrestin, Rémi Lemoine and Soulaïman Sakr

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 51, issue 11, pages 1878-1888
Published in print November 2010 | ISSN: 0032-0781
Published online October 2010 | e-ISSN: 1471-9053 | DOI:
Cloning and Characterization of a New Polyol Transporter (HbPLT2) in Hevea brasiliensis

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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Quebrachitol is a cyclic polyol and, along with sucrose, is one of the main sugars in Hevea latex. However, in contrast to sucrose, the mechanism and regulation of quebrachitol absorption is still unknown. Screening a latex-derived cDNA library using polyol transporter-specific probes, two full-length cDNAs were isolated, and named HbPLT1 and HbPLT2 (for Hevea brasiliensis polyol transporter 1 and 2, respectively). Their respective sequences exhibited close similarity with the previously cloned acyclic sugar polyol transporters, and shared the main features of the major facilitative superfamily. The functional activity of one of the cDNAs was determined by using an HbPLT2-complemented yeast strain. These strains displayed a marginal absorption of cyclic (inositol) and acyclic (mannitol and sorbitol) polyol but no absorption of sucrose, hexose and glycerol. Active absorption for xylitol was detected, and was competitively inhibited by quebrachitol. HbPLT1 and HbPLT2 expression patterns varied in response to different stimuli. Bark treatment with ethylene resulted in an early and significant up-regulation of HbPLT2 transcripts in laticifers as well as in inner bark cells, when compared with HbPLT1. Other treatments, especially mechanical wounding, strongly induced HbPLT2 transcripts. These data were consistent with the presence of ethylene and a wound-responsive regulatory cis-element on the sequence of the HbPLT2 promoter. All these findings together with those recently obtained for sucrose transporters and aquaporins are discussed in relation to the different roles for quebrachitol in Hevea brasiliensis.

Keywords: Hevea brasiliensis; Latex production; Polyol transporter; Quebrachitol

Journal Article.  6700 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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