Journal Article

The R-R-Type MYB-Like Transcription Factor, AtMYBL, is Involved in Promoting Leaf Senescence and Modulates an Abiotic Stress Response in Arabidopsis

Xia Zhang, Hyun-Woo Ju, Moon-Soo Chung, Ping Huang, Sung-Ju Ahn and Cheol Soo Kim

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 52, issue 1, pages 138-148
Published in print January 2011 | ISSN: 0032-0781
Published online November 2010 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcq180
The R-R-Type MYB-Like Transcription Factor, AtMYBL, is Involved in Promoting Leaf Senescence and Modulates an Abiotic Stress Response in Arabidopsis

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Functional analysis of a putative novel transcription factor Arabidopsis MYB-like protein designated AtMYBL, which contains two predicted DNA-binding domains, was performed. The physiological role of the R-R-type MYB-like transcription factor has not been reported in any plant. Analyses of an AtMYBL promoter–β-glucuronidase (GUS) construct revealed substantial gene expression in old leaves and induction of GUS activity by ABA and salt stress. AtMYBL-overexpressing plants displayed a markedly enhanced leaf senescence phenotype. Moreover, the ectopic expression of the AtMYBL gene was very significantly influential in senescence parameters including Chl content, membrane ion leakage and the expression of senescence-related genes. Although the seed germination rate was improved under ABA and saline stress conditions in the AtMYBL-overexpressing plants, decreased salt tolerance was evident compared with the wild type and atmybl RNA interference lines during later seedling growth when exposed to long-term salt stress, indicating that AtMYBL protein is able to developmentally regulate stress sensitivity. Furthermore, AtMYBL protein activated the transcription of a reporter gene in yeast. Green fluorescent protein-tagged AtMYBL was localized in the nuclei of transgenic Arabidopsis cells. Taken together, these results suggest that AtMYBL functions in the leaf senescence process, with the abiotic stress response implicated as a putative potential transcription factor.

Keywords: Abscisic acid; Leaf senescence; MYB-like family; Salt stress; Senescence-related gene; Transcription factor

Journal Article.  5567 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.