Journal Article

The Circadian Clock Modulates Water Dynamics and Aquaporin Expression in Arabidopsis Roots

Tomoyuki Takase, Haruki Ishikawa, Haruko Murakami, Jun Kikuchi, Kumi Sato-Nara and Hitoshi Suzuki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 52, issue 2, pages 373-383
Published in print February 2011 | ISSN: 0032-0781
Published online December 2010 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq198
The Circadian Clock Modulates Water Dynamics and Aquaporin Expression in Arabidopsis Roots

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We have developed a plant growth system to analyze water dynamics in the roots of a small model plant, Arabidopsis thaliana, by nuclear magnetic resonance (NMR) microscopic imaging. Using the two-dimensional slice technique, we obtained a series of images with high signal-to-noise ratio indicating the water distribution in the root. To demonstrate light regulation of water transport in the root and involvement of aquaporin gene expression, we visualized the distribution of water in Arabidopsis roots under various light conditions and compared the data with the expression profiles of two aquaporin genes. 1H-NMR imaging revealed that water content in Arabidopsis roots is lower in the light than in the dark. This diurnal variation in water content was clearly observed in the basal zone of the root. In addition, an autonomous rhythm of water dynamics was observed under continuous light (LL) and darkness (DD). However, the circadian oscillation in water dynamics was obscured in the early-flowering 3 (elf3) mutant under LL. The expression of both the aquaporin genes, AtPIP1;2 and AtPIP2;1, oscillated with the circadian rhythm under LL conditions in wild-type seedlings, but not in the elf3 mutant. These results demonstrate the advantages of our technique for monitoring water dynamics in roots of living Arabidopsis seedlings, and suggest that the circadian clock modulates water dynamics and aquaporin expression.

Keywords: Aquaporin; Arabidopsis thaliana; Circadian regulation; 1H-NMR imaging; Root; Water dynamics

Journal Article.  7036 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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