Journal Article

A Response Regulator Rre37 and an RNA Polymerase Sigma Factor SigE Represent Two Parallel Pathways to Activate Sugar Catabolism in a Cyanobacterium <i>Synechocystis</i> sp. PCC 6803

Miyuki Azuma, Takashi Osanai, Masami Yokota Hirai and Kan Tanaka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 52, issue 2, pages 404-412
Published in print February 2011 | ISSN: 0032-0781
Published online January 2011 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcq204
A Response Regulator Rre37 and an RNA Polymerase Sigma Factor SigE Represent Two Parallel Pathways to Activate Sugar Catabolism in a Cyanobacterium Synechocystis sp. PCC 6803

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Sugar catabolic genes are induced during nitrogen starvation in a cyanobacterium Synechocystis sp. PCC 6803, but the underlying regulatory mechanism still remains to be completely characterized. In this study, we showed by molecular genetics and transcriptome analyses that a response regulator Rre37 (encoded by sll1330), whose expression is enhanced by nitrogen depletion under the control of NtcA, activates transcript accumulation of sugar catabolic genes, such as gap1, pfkA (sll1196), glgP (slr1367) and glgX (slr1857), mainly during nitrogen starvation. Previously, we reported that a group-2 sigma factor SigE also positively regulates sugar catabolic genes in this strain. Phenotypic analyses using a single or double mutant lacking rre37 and/or sigE indicated that both SigE and Rre37 positively regulate sugar catabolic genes independently. These findings substantiated a regulatory network of sugar catabolic genes in this cyanobacterium.

Keywords: Cyanobacteria; Nitrogen starvation; NtcA; Response regulator; Sigma factor; Sugar catabolism

Journal Article.  5046 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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