Journal Article

Sublethal Cadmium Intoxication In <i>Arabidopsis thaliana</i> Impacts Translation at Multiple Levels

R. Sormani, E. Delannoy, S. Lageix, F. Bitton, E. Lanet, J. Saez-Vasquez, J. M. Deragon, J. P. Renou and C. Robaglia

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 52, issue 2, pages 436-447
Published in print February 2011 | ISSN: 0032-0781
Published online January 2011 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcr001
Sublethal Cadmium Intoxication In Arabidopsis thaliana Impacts Translation at Multiple Levels

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To study the impact of translational regulation during heavy metal poisoning, Arabidopsis thaliana cell cultures were submitted to sublethal cadmium stress. At the concentration used, cadmium had a minimal impact on the growth of the culture but induced an accumulation of high molecular weight polysomes without de novo production of new ribosomes together with a reduction of protein synthesis. In addition, cadmium stress induces phosphorylation of eukaryotic initiation factor 2α by GCN2 and, in planta, gcn2 mutants are more sensitive to cadmium stress, suggesting a role for this translational regulation mechanism in the response to cadmium stress. Microarray analysis of total and polysomal RNAs in control and cadmium-treated cells reveals a large class of genes for which a variation in total RNA abundance is not linked to a variation in polysomal loading, suggesting that transcription and translation are uncoupled and that these genes are not recruited at the initiation step of translation.

Keywords: Abiotic stress; Arabidopsis eIF2α; Cadmium; Polysome; Translation

Journal Article.  7531 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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