Journal Article

The Jasmonate-Responsive Element from the <i>ORCA3</i> Promoter from <i>Catharanthus roseus</i> is Active in Arabidopsis and is Controlled by the Transcription Factor AtMYC2

Gregory Montiel, Adel Zarei, Ana P. Körbes and Johan Memelink

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 52, issue 3, pages 578-587
Published in print March 2011 | ISSN: 0032-0781
Published online February 2011 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcr016
The Jasmonate-Responsive Element from the ORCA3 Promoter from Catharanthus roseus is Active in Arabidopsis and is Controlled by the Transcription Factor AtMYC2

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  • Molecular and Cell Biology
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Jasmonates are plant signaling molecules that play key roles in protection against certain pathogens and against insects by switching on the expression of genes encoding defense proteins including enzymes involved in the biosynthesis of toxic secondary metabolites. In Catharanthus roseus, the ethylene response factor (ERF) transcription factor ORCA3 controls the jasmonate-responsive activation of terpenoid indole alkaloid biosynthetic genes. ORCA3 gene expression is itself induced by jasmonate. Its promoter contains an autonomous jasmonate-responsive element (JRE). Here we describe the jasmonate-responsive activity of the JRE from the ORCA3 promoter in Arabidopsis thaliana. We found that it interacts in vitro and in vivo with the basic helix–loop–helix transcription factor AtMYC2. Analysis of JRE-mediated reporter gene expression in an atmyc2-1 mutant background showed that the activity was strictly dependent on AtMYC2.

Keywords: Arabidopsis thaliana; AtMYC2; Catharanthus roseus; Jasmonate-responsive element; ORCA3; Transcription factor

Journal Article.  6057 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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