Journal Article

Nitric Oxide Production Induced in Roots of <i>Lotus japonicus</i> by Lipopolysaccharide from <i>Mesorhizobium loti</i>

Ei-ichi Murakami, Maki Nagata, Yoshikazu Shimoda, Ken-ichi Kucho, Shiro Higashi, Mikiko Abe, Masahito Hashimoto and Toshiki Uchiumi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 52, issue 4, pages 610-617
Published in print April 2011 | ISSN: 0032-0781
Published online February 2011 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcr020
Nitric Oxide Production Induced in Roots of Lotus japonicus by Lipopolysaccharide from Mesorhizobium loti

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Lipopolysaccharide (LPS) is a bacterial molecule that induces nitric oxide (NO) production and triggers defense systems in plant–pathogen interactions. NO production is induced in the roots of Lotus japonicus after inoculation of the roots with its microsymbiont Mesorhizobium loti. However, the rhizobial molecule that induces NO production has not yet been identified. We investigated NO production in the roots of L. japonicus by treatment with LPS of M. loti. LPS was prepared by phenol–hot water extraction and separated into several fractions: polysaccharide, lipooligosaccharide, oligosaccharide and lipid A. In the roots of L. japonicus, NO production was observed with an NO-specific fluorescent dye 4, 10 and 24 h after treatment with each fraction of LPS. NO production was detected 4 h after treatment with all fractions. NO production was also detectable 24 h after treatment, except after treatment with the polysaccharide and oligosaccharide fractions. Expression of a class 1 hemoglobin gene and application of an NO scavenger showed that the treatment with LPS and LOS induced a similar response to inoculation with M. loti. These data suggest that LPS of M. loti induces NO production after inoculation with M. loti.

Keywords: Lipopolysaccharide; Lotus japonicus; Mesorhizobium loti; Nitric oxide; Symbiosis

Journal Article.  4115 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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