Journal Article

A C-Terminal Membrane Association Domain of Phototropin 2 is Necessary for Chloroplast Movement

Sam-Geun Kong, Takatoshi Kagawa, Masamitsu Wada and Akira Nagatani

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 54, issue 1, pages 57-68
Published in print January 2013 | ISSN: 0032-0781
Published online September 2012 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcs132
A C-Terminal Membrane Association Domain of Phototropin 2 is Necessary for Chloroplast Movement

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Phototropins (phot1 and phot2), plant-specific blue light receptor kinases, mediate a range of physiological responses in Arabidopsis, including phototropism, chloroplast photorelocation movement, stomatal opening and leaf flattening. Phototropins consist of two photoreceptive domains at their N-terminus, LOV1 (light, oxygen or voltage 1) and LOV2, and a serine/threonine kinase domain at their C-terminus. Here, we determined the molecular moiety for the membrane association of phototropins using the yeast CytoTrap and Arabidopsis protoplast systems. We then examined the physiological significance of the membrane association of phototropins. This detailed study with serial deletions narrowed down the association domain to a relatively small part of the C-terminal domain of phototropin. The functional analysis of phot2 deletion mutants in the phot2-deficient Adiantum and Arabidopsis mutants revealed that the ability to mediate the chloroplast avoidance response correlated well with phot2’s membrane association, especially with the Golgi apparatus. Taken together, our data suggest that a small part of the C-terminal domain of phototropins is necessary not only for membrane association but also for the physiological activities that elicit phototropin-specific responses.

Keywords: Arabidopsis thaliana; Blue light; Light signaling; Membrane association; Photoreceptor; Phototropin

Journal Article.  7536 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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