Journal Article

Overexpression of OsRecQl4 and/or OsExo1 Enhances DSB-Induced Homologous Recombination in Rice

Yong-Ik Kwon, Kiyomi Abe, Keishi Osakabe, Masaki Endo, Ayako Nishizawa-Yokoi, Hiroaki Saika, Hiroaki Shimada and Seiichi Toki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 53, issue 12, pages 2142-2152
Published in print December 2012 | ISSN: 0032-0781
Published online November 2012 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcs155
Overexpression of OsRecQl4 and/or OsExo1 Enhances DSB-Induced Homologous Recombination in Rice

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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During homologous recombination (HR)-mediated DNA double-strand break (DSB) repair in eukaryotes, an initial step is the creation of a 3′-single-stranded DNA (ssDNA) overhang via resection of a 5′ end. Rad51 polymerizes on this ssDNA to search for a homologous sequence, and the gapped sequence is then repaired using an undamaged homologous DNA strand as template. Recent studies in eukaryotes indicate that resection of the DSB site is promoted by the cooperative action of RecQ helicase family proteins: Bloom helicase (BLM) in mammals or Sgs1 in yeast, and exonuclease 1 (Exo1). However, the role of RecQ helicase and exonuclease during the 5′-resection process of HR in plant cells has not yet been defined. Here, we demonstrate that overexpression of rice proteins OsRecQl4 (BLM counterpart) and/or OsExo1 (Exo1 homolog) can enhance DSB processing, as evaluated by recombination substrate reporter lines in rice. These results could be applied to construct an efficient gene targeting system in rice.

Keywords: OsRecQl4; OsExo1; rice; DSB; HR; resection step

Journal Article.  5737 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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