Journal Article

The K<sup>+</sup>-Dependent Asparaginase, NSE1, is Crucial for Plant Growth and Seed Production in <i>Lotus japonicus</i>

Alfredo Credali, Margarita García-Calderón, Svend Dam, Jillian Perry, Antonio Díaz-Quintana, Martin Parniske, Trevor L. Wang, Jens Stougaard, José M. Vega and Antonio J. Márquez

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 54, issue 1, pages 107-118
Published in print January 2013 | ISSN: 0032-0781
Published online November 2012 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcs156
The K+-Dependent Asparaginase, NSE1, is Crucial for Plant Growth and Seed Production in Lotus japonicus

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The physiological role of K+-dependent and K+-independent asparaginases in plants remains unclear, and the contribution from individual isoforms during development is poorly understood. We have used reverse genetics to assess the phenotypes produced by the deficiency of K+-dependent NSE1 asparaginase in the model legume Lotus japonicus. For this purpose, four different mutants were identified by TILLING and characterized, two of which affected the structure and function of the asparaginase molecule and caused asparagine accumulation. Plant growth and total seed weight of mature mutant seeds as well as the level of both legumin and convicilin seed storage proteins were affected in the mutants. The mutants isolated in the present work are the first of their type in legumes and have enabled us to demonstrate the importance of asparagine and K+-dependent NSE1 asparaginase for nitrogen remobilization and seed production in L. japonicus plants.

Keywords: Asparaginase; Lotus japonicus; Nitrogen metabolism; Nitrogen remobilization; Reverse genetics; TILLING mutants

Journal Article.  5875 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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