Journal Article

OsDGL1, a Homolog of an Oligosaccharyltransferase Complex Subunit, is Involved in N-Glycosylation and Root Development in Rice

Cheng Qin, Yuanya Li, Jian Gan, Wenxia Wang, Huanhuan Zhang, Yu Liu and Ping Wu

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 54, issue 1, pages 129-137
Published in print January 2013 | ISSN: 0032-0781
Published online December 2012 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcs159
OsDGL1, a Homolog of an Oligosaccharyltransferase Complex Subunit, is Involved in N-Glycosylation and Root Development in Rice

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  • Biochemistry
  • Molecular and Cell Biology
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A leaky rice mutant was isolated from an ethylmethane sulfonate (EMS)-mutagenized rice library based on its short root phenotype. The map-based cloning results showed that the mutant was due to a point mutation in the intron of OsDGL1 (LOC_Os07g10830), which encodes the dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48 kDa subunit precursor. The mutation results in premature termination of protein synthesis. OsDGL1 is an ortholog of Arabidopsis DGL1, human OST48 and yeast WBP1, an essential protein subunit of the oligosaccharyltransferase (OST) complex, which is involved in N-glycosylation in eukaryotes. The leaky rice mutant, Osdgl1, displayed a change of matrix polysaccharides in its root cell wall, shorter root cell length, smaller root meristem and cell death in the root. Consistent with the known function of the OST complex in eukaryotes, the Osdgl1 mutation leads to a defect in N-glycosylation in the root. It was also found that reactive oxygen species (ROS) may be involved in this process.

Keywords: Cell death; Cell wall; N-glycosylation; Oligosaccharyltransferase; Rice; ROS

Journal Article.  5451 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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