Journal Article

Effects of Ozone Exposure on Nuclear Factor-κB Activation and Tumor Necrosis Factor-α Expression in Human Nasal Epithelial Cells

Brian G. Nichols, James S. Woods, Daniel L. Luchtel, Jeannette Corral and Jane Q. Koenig

in Toxicological Sciences

Volume 60, issue 2, pages 356-362
Published in print April 2001 | ISSN: 1096-6080
Published online April 2001 | e-ISSN: 1096-0929 | DOI: http://dx.doi.org/10.1093/toxsci/60.2.356
Effects of Ozone Exposure on Nuclear Factor-κB Activation and Tumor Necrosis Factor-α Expression in Human Nasal Epithelial Cells

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

In this study we investigated a possible mechanism of the human airway inflammatory response to inhaled ozone (O3). Cultures of human nasal epithelial (HNE) cells, initiated from excised nasal turbinates and grown on collagen-coated Transwell® tissue culture inserts, were exposed to 120, 240, or 500 ppb O3 for 3 h. An electron spin resonance (ESR) signal that changed with time suggested free radical production in HNE cells exposed to O3. Nuclear protein extracts were analyzed for the activated transcription factor NF-κB by electrophoretic mobility-shift assay (EMSA), and showed a small dose-response activation of NF-κB that coincided with O3-induced free radical production. Basal media were analyzed for the presence of tumor necrosis factor-α (TNF-α) using the enzyme-linked immunosorbent assay (ELISA). In cultures exposed to 120 ppb O3, the mean TNF-α concentration was not significantly different from those exposed to air. However, exposure to 240 and 500 ppb O3 significantly increased mean TNF-α expression, relative to controls, 16 h after exposure. These results support the hypothesis that the human airway epithelium plays a role in directing the inflammatory response to inhaled O3 via free radical-mediated NF-κB activation.

Keywords: airway; cell culture; cytokine; electron spin resonance; epithelial cell, free radical; inflammation; nasal cell; ozone; transcription factor; tumor necrosis factor.

Journal Article.  5025 words.  Illustrated.

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.