Journal Article

2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin Interacts with Endogenous Estradiol to Disrupt Prostate Gland Morphogenesis in Male Rat Fetuses

Barry G. Timms, Richard E. Peterson and Frederick S. vom Saal

in Toxicological Sciences

Volume 67, issue 2, pages 264-274
Published in print June 2002 | ISSN: 1096-6080
Published online June 2002 | e-ISSN: 1096-0929 | DOI: http://dx.doi.org/10.1093/toxsci/67.2.264
2,3,7,8-Tetrachlorodibenzo-p-dioxin Interacts with Endogenous Estradiol to Disrupt Prostate Gland Morphogenesis in Male Rat Fetuses

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Fetal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) interferes with normal development of the male reproductive system in rats and mice. We examined the effects of TCDD on the initial development of the urogenital system (urethra, prostate, and seminal vesicles) in male rat fetuses on gestation day (GD) 20. The number of prostatic buds and size of prostate glands as well as seminal vesicle size was determined by computer-assisted 3D reconstruction. Pregnant Holtzman rats received a single oral dose of TCDD (1 μg/kg) on GD 15. The intrauterine position (IUP) of male fetuses was identified based on the sex of adjacent fetuses: 2F males were located between 2 females and 2M males were located between 2 males. Control 2F males had elevated serum estradiol and larger prostates than control 2M males, which had elevated serum testosterone and larger seminal vesicles, confirming prior findings. There was no effect of TCDD on serum testosterone. TCDD significantly decreased the number of buds in the dorsocranial and dorsolateral regions of the urogenital sinus and overall prostate size, and was associated with a significant decrease in serum estradiol only in 2F males. In contrast, in 2M males both serum estradiol and the number and size of prostatic buds in these same regions of the prostate were unaffected by TCDD, although seminal vesicle size was reduced. These findings show that individual differences in gonadal steroid levels influence the response of the developing prostate to TCDD in male fetuses. In addition, these TCDD effects may be mediated in part by a decrease in serum estradiol levels.

Keywords: 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD); prostate development; intrauterine position; testosterone; estrogen; rat

Journal Article.  8777 words.  Illustrated.

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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