Journal Article

Assessment of Genotoxicity of Benzidine and Its Structural Analogues to Human Lymphocytes Using Comet Assay

Ssu-Ching Chen, Chih-Ming Kao, Mei-Han Huang, Ming-Kuei Shih, Ya-Lei Chen, Shiao-Ping Huang and Tsan-Zon Liu

in Toxicological Sciences

Volume 72, issue 2, pages 283-288
Published in print April 2003 | ISSN: 1096-6080
Published online April 2003 | e-ISSN: 1096-0929 | DOI: http://dx.doi.org/10.1093/toxsci/kfg026
Assessment of Genotoxicity of Benzidine and Its Structural Analogues to Human Lymphocytes Using Comet Assay

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Benzidine (BZ) and its six structural analogues (2-aminobiphenyl [2-ABP], 4-aminobiphenyl [4-ABP], 3,3′-diaminobenzidine [DABZ], 3,3′-dichlorobenzidine [DCBZ] 3,3′-dimethoxybenzidine [DEBZ], and 3,3′-dimthylbenzidine [DMBZ]) were examined for DNA damage in human lymphocytes using the alkaline comet assay. All the tested compounds showed a distinct disparity in their respective DNA-damaging capacities with an order of DABZ > BZ > DCBZ > 2-ABP > DEBZ > 4-ABP > DMBZ when lymphocytes were exposed to these chemicals for 2 h. Results show that the DNA-damaging effects of these compounds had no bearing on some physicochemical parameters including oxidation potentials, the energy differences between the lowest unoccupied molecular orbital and the highest occupied molecular orbital, ionization potentials, dipole moment, and relative partition coefficient. On the other hand, the free radical scavengers, including catalase, SOD, BHT, EDTA, and histidine exerted varying degrees of inhibitory effects on the DNA damage caused by benzidine. This suggests that genotoxicity in lymphocytes caused by benzidine proceeded via a reactive oxygen species (ROS)-mediated mechanism.

Keywords: benzidine; comet assay; ROS; scavengers

Journal Article.  4322 words.  Illustrated.

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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