Journal Article

The Laminin Binding Protein p40 Is Involved in Inducing Limb Abnormality of Mouse Fetuses as the Effects of Methoxyacetic Acid Treatment

Aceng Ruyani, Sri Sudarwati, Lien A. Sutasurya, Sony H. Sumarsono and Torsten Gloe

in Toxicological Sciences

Volume 75, issue 1, pages 148-153
Published in print September 2003 | ISSN: 1096-6080
Published online September 2003 | e-ISSN: 1096-0929 | DOI: http://dx.doi.org/10.1093/toxsci/kfg159
The Laminin Binding Protein p40 Is Involved in Inducing Limb Abnormality of Mouse Fetuses as the Effects of Methoxyacetic Acid Treatment

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This study is intended to characterize a protein that is linked with mouse limb teratogenicity as the effects of methoxyacetic acid (MAA) treatment. A single dose of MAA (10 mmol/kg body weight) was given by gavage on gestation day (GD) 11, whereas the control group were administered vehicle only. The pregnant mice were killed at 4 h after MAA treatment, and forelimb buds were isolated from both the control and treated group embryos. Proteins from forelimb buds GD 11 + 4 h, which were precipitated out using 40–60% ammonium sulfate, then were analyzed by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2-D SDS–PAGE) technique. The 2-D gels reveal one protein with 41.6 kDa and pI 6.4, which expression was downregulated after MAA treatment. Tentative protein identification via peptide mass database search and definitive protein identification via a primary sequence database search indicate that the protein matches exactly to 34/67 kDa laminin binding protein (LBP; P14206, SwissProt), which is encoded by p40 gene (MGI:105381). The identity was further verified by Western blotting with an antibody against the 67 kDa LBP. The results suggest that MAA treatment to pregnant mice downregulates the LBP-p40 in the forelimb buds.

Keywords: limb teratogenicity; methoxyacetic acid; 2-D SDS–PAGE; LBP-p40

Journal Article.  4052 words.  Illustrated.

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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