Journal Article

Sensitivity of Japanese Quail (<i>Coturnix japonica</i>), Common Pheasant (<i>Phasianus colchicus</i>), and White Leghorn Chicken (<i>Gallus gallus domesticus</i>) Embryos to <i>In Ovo</i> Exposure to TCDD, PeCDF, and TCDF

Andrew M. Cohen-Barnhouse, Matthew J. Zwiernik, Jane E. Link, Scott D. Fitzgerald, Sean W. Kennedy, Jessica C. Hervé, John P. Giesy, Steve Wiseman, Yinfei Yang, Paul D. Jones, Yi Wan, Brian Collins, John L. Newsted, Denise Kay and Steven J. Bursian

in Toxicological Sciences

Volume 119, issue 1, pages 93-103
Published in print January 2011 | ISSN: 1096-6080
Published online September 2010 | e-ISSN: 1096-0929 | DOI: http://dx.doi.org/10.1093/toxsci/kfq289
Sensitivity of Japanese Quail (Coturnix japonica), Common Pheasant (Phasianus colchicus), and White Leghorn Chicken (Gallus gallus domesticus) Embryos to In Ovo Exposure to TCDD, PeCDF, and TCDF

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

Egg injection studies were performed to confirm a proposed model of relative sensitivity of birds to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). In this model, species are classified as belonging to one of three categories of sensitivity based on amino acid substitutions in the ligand-binding domain of the aryl hydrocarbon receptor. Embryo lethality and relative potencies of 2,3,7,8-tetrachlorodibenzofuran (TCDF) and 2,3,4,7,8-pentachlorodibenzofuran (PeCDF) were compared with TCDD for Japanese quail (Coturnix japonica; least sensitive), Common pheasant (Phasianus colchicus; moderately sensitive), and White Leghorn chicken (Gallus gallus domesticus; most sensitive). Doses ranging from 0.044 to 37 pmol/g egg (0.015–12 ng/g egg) were injected into the air cell of eggs prior to incubation. LD50 (95% confidence intervals) values, based on rate of hatching for TCDD, PeCDF, and TCDF, were 30 (25–36), 4.9 (2.3–9.2), and 15 (11–24) pmol/g egg for the quail, 3.5 (2.3–6.3), 0.61 (0.28–1.2), and 1.2 (0.62–2.2) pmol/g egg for pheasant, and 0.66 (0.47–0.90), 0.75 (0.64–0.87), and 0.33 (0.23–0.45) pmol/g egg for chicken, respectively. LD50-based relative potencies of PeCDF and TCDF were 6.1 and 2.0 for quail, 5.7 and 2.9 for pheasant, and 0.88 and 2.0 for chicken, respectively. TCDD was not the most potent compound among the species tested, with PeCDF and TCDF being more potent than TCDD in the quail and pheasant. TCDF was the most potent in chicken. Species sensitivity was as expected for TCDD and TCDF, whereas for PeCDF, the chicken and pheasant were similar in sensitivity and both were more sensitive than the quail. Results from companion in vitro studies are generally similar to those reported here with a few exceptions.

Keywords: TCDD; PeCDF; TCDF; egg; bird; toxic equivalency factor; relative potency

Journal Article.  5969 words.  Illustrated.

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.