Journal Article

Persistent DNA Damage Measured by Comet Assay of Sprague Dawley Rat Lung Cells after Five Days of Inhalation Exposure and 1 Month Post-Exposure to Dispersed Multi-Wall Carbon Nanotubes (MWCNTs) Generated by New MWCNT Aerosol Generation System

Jin Sik Kim, Jae Huyck Sung, Kyung Seuk Song, Ji Hyun Lee, Sun Man Kim, Gun Ho Lee, Kang Ho Ahn, Jong Seong Lee, Jae Hoon Shin, Jung Duck Park and Il Je Yu

in Toxicological Sciences

Volume 128, issue 2, pages 439-448
Published in print August 2012 | ISSN: 1096-6080
Published online April 2012 | e-ISSN: 1096-0929 | DOI: http://dx.doi.org/10.1093/toxsci/kfs161
Persistent DNA Damage Measured by Comet Assay of Sprague Dawley Rat Lung Cells after Five Days of Inhalation Exposure and 1 Month Post-Exposure to Dispersed Multi-Wall Carbon Nanotubes (MWCNTs) Generated by New MWCNT Aerosol Generation System

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Carbon nanotubes (CNTs) have specific physico-chemical properties that are useful for the electronics, automotive, and construction industries. Yet, despite their many advantages, there is a current lack of available information on the human health and environmental hazards of CNTs. For this reason, the current study investigated the inhalation toxicity potential of multiwall CNTs (MWCNTs). Eight-week-old rats were divided into four groups (10 rats in each group), the fresh-air control (0mg/m3), low-concentration group (0.16mg/m3), middle-concentration group (0.34mg/m3), and high-concentration group (0.94mg/m3), and the whole body was exposed to MWCNTs for 5 days (6h/day). Lung cells were then isolated from five rats in each group on day 0 and 1 month after the 5-day exposure, respectively. The MWCNTs were generated by a newly designed generation system, and the MWCNT concentrations in the exposure chambers monitored in accordance with National Institute for Occupational Safety and Health (NIOSH) 0500 using a membrane filter. The MWCNTs were also sampled for an elemental carbon concentration analysis using a glass filter. The animals exhibited no significant body weight changes, abnormal clinical signs, or mortality during the experiment. A single-cell gel electrophoresis assay (Comet assay) was conducted to determine the DNA damage in lung cells obtained from the right lung. As a result, the Olive tail moments were 23.00±1.76, 30.39±1.96, 22.96±1.26, and 33.98±2.21 for the control, low-, middle-, and high-concentration groups, respectively, on day 0 postexposure. Meanwhile, 1 month postexposure, the Olive tail moments were 25.00±2.71, 28.39±3.55, 22.56±1.36, and 31.97±3.16 for the control, low-, middle-, and high-concentration groups, respectively. Thus, the MWCNTs caused a statistically significant increase in lung DNA damage at high concentration (0.94mg/m3) when compared with the negative control group on day 0 and 1 month postexposure.

Keywords: CNTs; MWCNTs; inhalation; single-cell gel electrophoresis assay (Comet assay); lung DNA damage

Journal Article.  5739 words.  Illustrated.

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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