Journal Article

<i>In vitro</i> regeneration of plantlets from mature embryos of <i>Pinus ayacahuite</i>

Francisco Saborio, William S. Dvorak, Jeffrey K. Donahue and Trevor A. Thorpe

in Tree Physiology

Volume 17, issue 12, pages 787-796
Published in print December 1997 | ISSN: 0829-318X
Published online December 1997 | e-ISSN: 1758-4469 | DOI:
In vitro regeneration of plantlets from mature embryos of Pinus ayacahuite

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A plantlet regeneration protocol was developed for Pinus ayacahuite var. ayacahuite (Ehrenb.). Embryos from mature seeds from ten provenances were cultured in a 16-h photoperiod for 3 days on a medium containing 30 mM sucrose and 0.7% agar. Cotyledons from these embryos were subcultured onto MCM medium (Bornman 1983) supplemented with 50 μM N6-benzyladenine and 90 mM sucrose for 2 weeks. Bud development and shoot elongation were maximized by subculturing the explants on half strength AE medium (von Arnold and Ericksson 1981), supplemented with 60 mM sucrose and 0.05% activated charcoal every 30 days. Seed source had a significant effect on the responses of the embryos to the bud induction protocol. For the provenance with the best response to bud induction, about 79% of the cultured cotyledons formed buds, and each cotyledon formed a mean of 9.1 buds, so that about 70 shoots could be induced from each seed. The best rooting response (40% rooting) was obtained by treating the shoots for 8 h with 100 μM naphthalene acetic acid.

Keywords: conifer; explant; hyperhydricity; micropropagation; organogenesis; pine; provenances

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry

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