Journal Article

Effects of nitrogen source on growth and activity of nitrogen-assimilating enzymes in Douglas-fir seedlings

J. P. Bedell, M. Chalot, A. Garnier and B. Botton

in Tree Physiology

Volume 19, issue 3, pages 205-210
Published in print March 1999 | ISSN: 0829-318X
Published online March 1999 | e-ISSN: 1758-4469 | DOI: http://dx.doi.org/10.1093/treephys/19.3.205
Effects of nitrogen source on growth and activity of nitrogen-assimilating enzymes in Douglas-fir seedlings

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Douglas-fir (Pseudotsuga menziesii(Mirb.) Franco) seedlings were grown for 68 days in a growth chamber in nutrient solutions with ammonium, nitrate or ammonium nitrate as the nitrogen source. Among the nitrogen sources tested, whole-seedling biomass, relative growth rate (RGR), root and shoot elongation, and number of lateral roots, were greatest in seedlings grown with ammonium. In the absence of nitrogen, plant growth and formation of lateral roots were poor. Initially, glutamine synthetase, NAD-glutamate dehydrogenase and aspartate aminotransferase activities were high in young roots and shoots, but all three enzymatic activities decreased after one month of culture. In root apices, glutamine synthetase and aspartate aminotransferase activities were higher than NAD-glutamate dehydrogenase activity. Enzymatic activities were often higher in ammonium-fed seedlings than in seedings supplied with the other forms of nitrogen. Activities of all three enzymes were significantly reduced in seedlings grown in the absence of nitrogen. The beneficial effect of ammonium is discussed on the basis of its involvement in the assimilation pathways of Douglas-fir.

Keywords: ammonium; aspartate aminotransferase; glutamate dehydrogenase; glutamine synthetase; nitrate; nitrogen metabolism; Pseudotsuga menziesii

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry

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