Journal Article

Characterization of genes for novel thaumatin-like proteins in <i>Cryptomeria japonica</i>

Norihiro Futamura, Naoki Tani, Yoshihiko Tsumura, Nobuyoshi Nakajima, Masahiko Sakaguchi and Kenji Shinohara

in Tree Physiology

Volume 26, issue 1, pages 51-62
Published in print January 2006 | ISSN: 0829-318X
Published online January 2006 | e-ISSN: 1758-4469 | DOI: http://dx.doi.org/10.1093/treephys/26.1.51
Characterization of genes for novel thaumatin-like proteins in Cryptomeria japonica

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Thaumatin-like proteins (TLPs) are induced by a variety of phytopathogens in many plants and several TLPs are allergenic. Previously, we isolated three TLP-encoding cDNAs (Cry j 3.1, Cry j 3.2 and Cry j 3.3) from a cDNA library derived from the pollen of Cryptomeria japonica D. Don. Here, we describe three new TLP cDNAs (Cry j 3.4, Cry j 3.5 and Cry j 3.6). We compared the sequences, the genetic map location and the expression patterns of the Cry j 3 genes. The amino acid sequence predicted from Cry j 3.5 exhibits only limited similarity to those predicted from the other Cry j 3 genes. Linkage analysis showed that the Cry j 3.1 to Cry j 3.4 genes are located in the same linkage group, but Cry j 3.5 is located in a different group. Organ-specificity and induction by stresses and plant hormones differed among the Cry j 3 mRNAs. In pollen grains, the Cry j 3.5 mRNA expression level was higher than that of the other Cry j 3 genes. Exposure to UV-B and salt stress induced expression of Cry j 3.1. The ethylene-releasing compound ethephon strongly induced expression of Cry j 3.4. Salt stress and salicylic acid also induced expression of Cry j 3.4. Abscisic acid weakly induced expression of Cry j 3.5. Arachidonic acid strongly induced expression of Cry j 3.4 and Cry j 3.6, and weakly induced that of Cry j 3.3, whereas expression of Cry j 3.1 and Cry j 3.5 was unaffected. These results suggest that the roles of TLPs and the cascades that regulate their expression differ among the members of the TLP family in C. japonica.

Keywords: gene expression; pollen; PR-5 protein; stress response; sugi (Cryptomeria japonica D. Don)

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry

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